HSV-LSL-tdtomato-2a-TK(H356)[Herpes Simplex Virus (HSV)]:
Herpes simplex virus (HSV) based on the H129 strain has been widely used as an anterograde tracer. The addition of fluorescent protein-modified HSV can not only effectively mark the connections between different brain regions, but also the connections between the surrounding and the center.
Thymidine nucleotide kinase (TK) is an important part of HSV replication after nerve cell infection. In the presence of TK, HSV can replicate and express target genes in cells, and its progeny viruses will be transported to the synapse, and then through the synapse to enter downstream neurons, thereby achieving multi-synaptic tracking. After deleting TK (deleting TK), HSV-ΔTK can infect neurons but cannot replicate. We know that only viruses that can replicate have the ability to cross synapses, so HSV-ΔTK cannot cross synapses. However, if we provide AAV virus as an assistant for expressing TK protein, HSV-ΔTK can achieve anterograde single synaptic tracking.
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